邹登朗，陈涛，李洪梅，陈晨，赵景阳，李玉林*. Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. Journal of Separation Science, 2016, 39, 1278-1285. IF=2.737

　　该研究首先利用响应面优化了三种目标化合物（诃子酸、诃黎勒酸、鞣花酸）的超声提取方法，在优化的条件下，三种目标化合物得到了很好的富集。然后，粗提物未经任何处理，直接采用高速逆流色谱进行分离得到了诃子酸、诃黎勒酸和鞣花酸，纯度均在98%以上。本方法简单、高效，非常适合诃子酸、诃黎勒酸和鞣花酸对照品的制备。

　　Abstract: This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound assisted extraction and high-speed counter-current chromatography. The ultrasound assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by 1H NMR spectroscopy.