耿丹丹,迟晓峰^*,董琦,胡风祖^*,Antioxidants screening in Limonium aureum by optimized on-line HPLC–DPPH assay,Industrial Crops and Products,2015,67:492-497.

　　该研究首先利用响应面法优化了HPLC-DPPH的条件，并在此条件下筛选出了黄花补血草中7种具有抗氧化作用的化合物。利用HPLC-MS技术鉴定出黄花补血草中具有抗氧化作用的化合物为杨梅酮-3-O-β-D-(6“-O-没食子酰基)-吡喃葡萄糖苷、杨梅素-3-O-葡萄糖苷、杨梅苷、圣草素-7-O-葡萄糖苷、杨梅酮、圣草素和高圣草素。

　　Abstract：Limonium aureum (Linn.) is a traditional Chinese medicine with strong antioxidant activity. To search for antioxidants in L. aureum, an optimized, on-line high performance liquid chromatography (HPLC) method with DPPH (1,1-diphenyl-2-picrylhydrazyl) for the detection of radical scavenging ability was developed. Response surface methodology (RSM) was used to determine the best combination of experimental conditions, such as DPPH concentration, DPPH velocity and the reaction tank length. The optimized method uses a DPPH concentration of 25.0 g/mL, DPPH velocity of 0.45 mL/min and a 15.0 m long reaction tank. The highly sensitive, optimized method can not only be used for the detection of antioxidants in plants, but can also be coupled with mass spectrometry (MS) to obtain the mass-to-charge ratios of chemical species corresponding to the different peaks in the HPLC profiles. Seven antioxidants were identified in L. aureum using the optimized method, including myricetin-3-O-β-d-(6”-O-galloyl)-glucopyranoside, myricetin-3-O-glucoside, myricitrin, eriodictyol-7-O-glucoside, myricetin, eriodictyol and homoeridictyol.